ELISA/assay

Human Stanniocalcin-1, STC1 ELISA Kit

MBS946255

48-Strip-Wells

510 USD

ELISA Kit

Human Stanniocalcin-1, STC1 ELISA Kit

stanniocalcin 1

Human Stanniocalcin-1 (STC1) ELISA kit; STC; ; stanniocalcin 1

stanniocalcin-1; Stanniocalcin-1; stanniocalcin-1; stanniocalcin 1

STC1

STC1; STC1; STC; STC; STC-1

STC1_HUMAN

Human

247

This assay has high sensitivity and excellent specificity for detection of human STC1. No significant cross-reactivity or interference between human STC1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.88 ng/ml -120 ng/ml. Sensitivity: The minimum detectable dose of human STC1 is typically less than 0.47 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for STC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4507265

NP_003146.1

NM_003155.2

P52823

27,621 Da

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers. [provided by RefSeq, Jul 2008]

STC1: Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia. Belongs to the stanniocalcin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p21.2. Cellular Component: extracellular space; apical plasma membrane; cytoplasm; nucleus. Molecular Function: hormone activity. Biological Process: cellular calcium ion homeostasis; ossification; negative regulation of calcium ion transport; response to vitamin D; decidualization; endothelial cell morphogenesis; negative regulation of cell migration; embryo implantation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800