Human CD9 antigen, CD9 ELISA Kit | MyBioSource MBS946197 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human CD9 antigen, CD9 ELISA Kit

catalog :

MBS946197

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS946197

products type :

ELISA Kit

products full name :

Human CD9 antigen, CD9 ELISA Kit

products short name :

CD9 molecule

products name syn :

Human CD9 antigen (CD9) ELISA kit; 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; MRP-1; P24; TSPAN29; 5H9 antigen; CD9 antigen; CD9 antigen (p24) ; OTTHUMP00000041576; antigen defined by monoclonal antibody 602-29; growth-inhibiting gene 2 protei; CD9 molecule

other names :

CD9 antigen; CD9 antigen; CD9 antigen; 5H9 antigen; tetraspanin-29; BA-2/p24 antigen; CD9 antigen (p24); leukocyte antigen MIC3; motility related protein-1; cell growth-inhibiting gene 2 protein; CD9 molecule; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD_antigen: CD9

products gene name :

CD9

other gene names :

CD9; CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29; MIC3; TSPAN29; GIG2; MRP-1; Tspan-29

uniprot entry name :

CD9_HUMAN

reactivity :

Human

sequence length :

228

specificity :

This assay has high sensitivity and excellent specificity for detection of human CD9. No significant cross-reactivity or interference between human CD9 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human CD9 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4502693

ncbi acc num :

NP_001760.1

ncbi gb acc num :

NM_001769.3

uniprot acc num :

P21926

ncbi mol weight :

25,416 Da

ncbi pathways :

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); A6b1 And A6b4 Integrin Signaling Pathway (138008)

ncbi summary :

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

uniprot summary :

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.3. Cellular Component: extracellular space; platelet alpha granule membrane; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle; external side of plasma membrane. Molecular Function: integrin binding; protein binding. Biological Process: platelet activation; fusion of sperm to egg plasma membrane; pathogenesis; multicellular organism reproduction; paranodal junction assembly; negative regulation of cell proliferation; platelet degranulation; single fertilization; brain development; cell motility; oligodendrocyte development; blood coagulation; cell adhesion; response to water deprivation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage