ELISA/assay

Mouse C-C chemokine receptor type 2, CCR2 ELISA Kit

MBS946089

48-Strip-Wells

510 USD

ELISA Kit

Mouse C-C chemokine receptor type 2, CCR2 ELISA Kit

chemokine (C-C motif) receptor 2

Mouse C-C chemokine receptor type 2 (CCR2) ELISA kit; CC-CKR-2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R; MCP-1 receptor; chemokine (C-C) receptor 2; monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor; chemokine (C-C motif) receptor 2

C-C chemokine receptor type 2; C-C chemokine receptor type 2; C-C chemokine receptor type 2; CCR-2; C-C CKR-2; MIP-1 alphaR; MCP-1 receptor; JE/FIC receptor; chemokine (C-C) receptor 2; chemoattractant protein-1 receptor; C-C CHEMOKINE RECEPTOR TYPE 2 (C-C CKR-2) (CC-CKR-2) (CCR-2) (CCR2) (JE/FIC RECEPTOR) (MCP-1 RECEPTOR); chemokine (C-C motif) receptor 2; JE/FIC receptor; MCP-1 receptor; CD_antigen: CD192

CCR2

Ccr2; Ccr2; Ckr2; Ccr2a; Ccr2b; Ckr2a; Ckr2b; mJe-r; Cmkbr2; Cc-ckr-2; Cmkbr2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2

CCR2_MOUSE

Mouse

373

This assay has high sensitivity and excellent specificity for detection of mouse CCR2. No significant cross-reactivity or interference between mouse CCR2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: The minimum detectable dose of mouse CCR2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCR2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6753466

NP_034045.1

NM_009915.2

P51683

42,783 Da

Chemokine Receptors Bind Chemokines Pathway (575428); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (575426); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (575454); GPCR Downstream Signaling Pathway (575452); GPCR Ligand Binding Pathway (575425); GPCRs, Class A Rhodopsin-like Pathway (198308)

CCR2: a seven transmembrane protein closely related to CCR1. Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. Transduces a signal by increasing the intracellular calcium ions level. Alternative co receptor with CD4 for HIV-1 infection. Two splice-variant isoforms have been described. Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral. Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: G-protein coupled receptor activity; signal transducer activity; protein binding; C-C chemokine receptor activity; cytokine binding; chemokine receptor activity. Biological Process: positive regulation of T-helper 1 type immune response; sensory perception of pain; chemotaxis; signal transduction; regulation of cell migration; monocyte chemotaxis; hemopoiesis; angiogenesis; cellular homeostasis; inflammatory response; healing during inflammatory response; positive regulation of tumor necrosis factor biosynthetic process; cytokine and chemokine mediated signaling pathway; humoral immune response; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of interferon-gamma production; negative regulation of T-helper 2 type immune response; positive regulation of interleukin-2 production; cellular defense response; positive regulation of alpha-beta T cell proliferation; immune response; blood vessel remodeling; positive regulation of T cell activation; negative regulation of eosinophil degranulation; positive regulation of T-helper 1 cell differentiation; apoptotic cell clearance; positive regulation of inflammatory response

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800