ELISA/assay

Human Melanotransferrin, MFI2 ELISA Kit

MBS946083

48-Strip-Wells

510 USD

ELISA Kit

Human Melanotransferrin, MFI2 ELISA Kit

antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5

Human Melanotransferrin (MFI2) ELISA kit; CD228; FLJ38863; MAP97; MGC4856; MTF1; melanoma-associated antigen p97; melanoma-associated antigen p97; isoform 2; melanotransferrin; membrane-bound transferrin-like protein; antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5

melanotransferrin isoform 1; Melanotransferrin; melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2; antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5; Melanoma-associated antigen p97; CD_antigen: CD228

MFI2

MFI2; MFI2; MTF1; CD228; MAP97; MAP97

TRFM_HUMAN

Human

738

This assay has high sensitivity and excellent specificity for detection of Human MFI2. No significant cross-reactivity or interference between Human MFI2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 2: Anion transport. Protein Biological Process 3: Ion transport

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for melanotransferrin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any melanotransferrin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for melanotransferrin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of melanotransferrin bound in the initial step. The color development is stopped and the intensity of the color is measured.

134244281

NP_005920.2

NM_005929.5

P08582

80,215 Da

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 3q28-q29. Cellular Component: anchored to plasma membrane; cell surface; integral to plasma membrane. Molecular Function: protein binding; ferric iron binding; iron ion binding. Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800