Human ProSAAS, PCSK1N ELISA Kit | MyBioSource MBS946045 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ProSAAS, PCSK1N ELISA Kit

catalog :

MBS946045

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS946045

products type :

ELISA Kit

products full name :

Human ProSAAS, PCSK1N ELISA Kit

products short name :

proprotein convertase subtilisin/kexin type 1 inhibitor

products name syn :

Human ProSAAS (PCSK1N) ELISA kit; PROSAAS; SAAS; granin-like neuroendocrine peptide; proprotein convertase subtilisin/kexin type 1 inhibitor

other names :

proSAAS preproprotein; ProSAAS; proSAAS; pro-SAAS; proprotein convertase 1 inhibitor; granin-like neuroendocrine peptide; proprotein convertase subtilisin/kexin type 1 inhibitor; Proprotein convertase subtilisin/kexin type 1 inhibitor; Proprotein convertase 1 inhibitor; pro-SAASCleaved into the following 7 chains:KEP; Big SAAS; b-SAAS; Little SAAS; l-SAASAlternative name(s):N-proSAAS

products gene name :

PCSK1N

other gene names :

PCSK1N; PCSK1N; SAAS; PROSAAS; Proprotein convertase 1 inhibitor; b-SAAS; l-SAAS; b-PEN-LEN; l-LEN; b-LEN

uniprot entry name :

PCSK1_HUMAN

reactivity :

Human

sequence length :

260

specificity :

This assay has high sensitivity and excellent specificity for detection of human PCSK1N. No significant cross-reactivity or interference between human PCSK1N and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human PCSK1N is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCSK1N has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCSK1N present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK1N is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK1N bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

7019519

ncbi acc num :

NP_037403.1

ncbi gb acc num :

NM_013271.4

uniprot acc num :

Q9UHG2

ncbi mol weight :

27,372 Da

ncbi summary :

The protein encoded by this gene functions as an inhibitor of prohormone convertase 1, which regulates the proteolytic cleavage of neuroendocrine peptide precursors. The proprotein is further processed into multiple short peptides. A polymorphism within this gene may be associated with obesity. [provided by RefSeq, Aug 2013]

uniprot summary :

PCSK1N: May function in the control of the neuroendocrine secretory pathway. Proposed be a specific endogenous inhibitor of PCSK1. ProSAAS and Big PEN-LEN, both containing the C-terminal inhibitory domain, but not the further processed peptides reduce PCSK1 activity in the endoplasmic reticulum and Golgi. It reduces the activity of the 84 kDa form but not the autocatalytically derived 66 kDa form of PCSK1. Subsequent processing of proSAAS may eliminate the inhibition. Slows down convertase-mediated processing of proopiomelanocortin and proenkephalin. May control the intracellular timing of PCSK1 rather than its total level of activity. The function of the processed secreted peptides is not known. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: Xp11.23. Cellular Component: extracellular space; trans-Golgi network; secretory granule. Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; receptor binding. Biological Process: response to dietary excess; neuropeptide signaling pathway; peptide hormone processing; response to cold

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage