ELISA/assay

Human Mitochondrial uncoupling protein 3, UCP3 ELISA Kit

MBS945861

48-Strip-Wells

510 USD

ELISA Kit

Human Mitochondrial uncoupling protein 3, UCP3 ELISA Kit

uncoupling protein 3 (mitochondrial, proton carrier)

Human Mitochondrial uncoupling protein 3 (UCP3) ELISA kit; SLC25A9; Uncoupling protein-3; uncoupling protein 3; uncoupling protein 3 (mitochondrial; proton carrier)

mitochondrial uncoupling protein 3 isoform UCP3L; Mitochondrial uncoupling protein 3; mitochondrial uncoupling protein 3; solute carrier family 25 member 9; uncoupling protein 3 (mitochondrial, proton carrier); Solute carrier family 25 member 9

UCP3

UCP3; UCP3; SLC25A9; SLC25A9; UCP 3

UCP3_HUMAN

Human

312

This assay has high sensitivity and excellent specificity for detection of human UCP3. No significant cross-reactivity or interference between human UCP3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 39 pg/ml -2500 pg/ml. Sensitivity: The minimum detectable dose of human UCP3 is typically less than 9.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UCP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4507807

NP_003347.1

NM_003356.3

P55916

34,216 Da

Diurnally Regulated Genes With Circadian Orthologs Pathway (198813); Electron Transport Chain Pathway (198860); Energy Metabolism Pathway (198907); Metabolism Pathway (477135); Mitochondrial Uncoupling Proteins Pathway (160963); Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway (105921); The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway (477137); The Fatty Acid Cycling Model Pathway (160964); The Proton Buffering Model Pathway (160965)

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.[provided by RefSeq, Nov 2008]

UCP3: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance. Defects in UCP3 may be involved in obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Mitochondrial. Chromosomal Location of Human Ortholog: 11q13.4. Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane. Molecular Function: protein binding; transporter activity; oxidative phosphorylation uncoupler activity. Biological Process: response to superoxide; response to glucocorticoid stimulus; respiratory gaseous exchange; fatty acid metabolic process; mitochondrial transport; cellular response to hormone stimulus; response to insulin stimulus; cellular metabolic process; proton transport; response to hypoxia; response to activity; response to cold; lipid metabolic process; response to nutrient; aging. Disease: Obesity

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800