catalog number :
MBS945851
products type :
ELISA Kit
products full name :
Monkey Hemoglobin subunit beta, HBB ELISA Kit
products short name :
hemoglobin, beta
products name syn :
Monkey Hemoglobin subunit beta (HBB) ELISA kit; CD113t-C; beta-globin; beta globin; beta globin chain; hemoglobin beta chain; hemoglobin; beta
other names :
Hemoglobin subunit beta; Hemoglobin subunit beta; Beta-globin; Hemoglobin beta chain
uniprot entry name :
HBB_MACMU
specificity :
This assay has high sensitivity and excellent specificity for detection of Monkey HBB. No significant cross-reactivity or interference between Monkey HBB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Lysate for RBC. Assay Type: Competitive. Detection Range: 7.81 ng/ml-500 ng/ml. Sensitivity: 3.9 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 2: Anion transport. Protein Biological Process 3: Oxygen transport
products description :
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for HBB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated HBB. A competitive inhibition reaction is launched between HBB (Standards or samples) and biotin-conjugated HBB with the pre-coated HBB antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substmonkeye solution is added to the wells and color develops in opposite to the amount of HBB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
15,997 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Involved in oxygen transport from the lung to the various peripheral tissues.
size4 :
10x96-Strip-Wells