Rat Pancreatic alpha-amylase, AMY2A ELISA Kit | MyBioSource MBS945823 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Pancreatic alpha-amylase, AMY2A ELISA Kit

catalog :

MBS945823

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS945823

products type :

ELISA Kit

products full name :

Rat Pancreatic alpha-amylase, AMY2A ELISA Kit

products short name :

[amylase, alpha 2A (pancreatic)]

products name syn :

[Rat Pancreatic alpha-amylase (AMY2A) ELISA kit; AMY2; AMY2B; PA; 1; 4-alpha-D-glucan glucanohydrolase; alpha-amylase; amylase; pancreatic; alpha-2A; found in the pancreas; glycogenase; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase; alpha 2A (pancreatic)]

other names :

[pancreatic alpha-amylase; Pancreatic alpha-amylase; pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase; amylase, alpha 2A (pancreatic); 1,4-alpha-D-glucan glucanohydrolase]

products gene name :

[AMY2A]

other gene names :

[AMY2A; AMY2A; PA; AMY2; AMY2B; PA]

uniprot entry name :

AMYP_HUMAN

reactivity :

Rat

sequence length :

511

specificity :

This assay has high sensitivity and excellent specificity for detection of rat AMY2A. No significant cross-reactivity or interference between rat AMY2A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich (Quantitative). Detection Range: 3.12 mIU/ml-200 mIU/ml. Sensitivity: 0.78 mIU/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMY2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMY2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMY2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMY2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4502085

ncbi acc num :

NP_000690.1

ncbi gb acc num :

NM_000699.2

uniprot acc num :

P04746

ncbi mol weight :

57,707 Da

ncbi pathways :

Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); Digestion Of Dietary Carbohydrate Pathway (106197); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Starch And Sucrose Metabolism Pathway (82974); Starch And Sucrose Metabolism Pathway (344)

ncbi summary :

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

uniprot summary :

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]. Protein type: Secreted, signal peptide; Secreted; Hydrolase; Carbohydrate Metabolism - starch and sucrose; EC 3.2.1.1. Chromosomal Location of Human Ortholog: 1p21. Cellular Component: extracellular space; extracellular region. Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding. Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage