ELISA/assay

Human Huntingtin, HTT ELISA Kit

MBS945813

96 Strip Wells

760 USD

ELISA Kit

Human Huntingtin, HTT ELISA Kit

huntingtin

Human Huntingtin (HTT) ELISA kit; HD; IT15; ; huntingtin

huntingtin; Huntingtin; huntingtin; huntington disease protein; huntingtin; Huntington disease protein; HD protein

HTT

HTT; HTT; HD; IT15; HD; IT15; HD protein

HD_HUMAN

Human

3144

This assay has high sensitivity and excellent specificity for detection of Human HTT. No significant cross-reactivity or interference between Human HTT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 12.5 pg/ml-800 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.125 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HTT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTT bound in the initial step. The color development is stopped and the intensity of the color is measured.

90903231

NP_002102.4

NM_002111.7

P42858

347,603 Da

Direct P53 Effectors Pathway 137939!!EGFR1 Signaling Pathway 198782!!Huntington's Disease Pathway 83100!!Huntington's Disease Pathway 512

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2008]

Function: May play a role in microtubule-mediated transport or vesicle function.

96 Strip Wells

760 USD