ELISA/assay

Human Carbonic anhydrase 1, CA1 ELISA Kit

MBS945790

48-Strip-Wells

510 USD

ELISA Kit

Human Carbonic anhydrase 1, CA1 ELISA Kit

carbonic anhydrase I

Human Carbonic anhydrase 1 (CA1) ELISA kit; Car1; carbonic dehydratase; carbonic anhydrase I

carbonic anhydrase 1 isoform a; Carbonic anhydrase 1; carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; epididymis secretory protein Li 11; carbonic anhydrase I; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I

CA1

CA1; CA1; CAB; CA-I; Car1; HEL-S-11; CAB; CA-I

CAH1_HUMAN

Human

261

This assay has high sensitivity and excellent specificity for detection of Human CA1. No significant cross-reactivity or interference between Human CA1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.9 ng/ml-60 ng/ml. Sensitivity: 0.225 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

192447430

NP_001122301.1

NM_001128829.3

P00915

28,870 Da

C-MYB Transcription Factor Network Pathway (138073)

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family. Protein type: Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen. Chromosomal Location of Human Ortholog: 8q21.2. Cellular Component: cytosol. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; one-carbon compound metabolic process

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800