ELISA/assay

Human Zona pellucida sperm-binding protein 3, ZP3 ELISA Kit

MBS945658

48-Strip-Wells

510 USD

ELISA Kit

Human Zona pellucida sperm-binding protein 3, ZP3 ELISA Kit

zona pellucida glycoprotein 3 (sperm receptor)

Human Zona pellucida sperm-binding protein 3 (ZP3) ELISA kit; ZP3A; ZP3B; ZPC; zona pellucida glycoprotein 3; zona pellucida glycoprotein 3A (sperm receptor) ; zona pellucida glycoprotein 3B; zona pellucida protein C; zona pellucida sperm-binding protein 3; zona pellucida glycoprotein 3 (sperm receptor)

zona pellucida sperm-binding protein 3 isoform 1; Zona pellucida sperm-binding protein 3; zona pellucida sperm-binding protein 3; ZP3A/ZP3B; sperm receptor; zona pellucida protein C; zona pellucida glycoprotein 3B; zona pellucida glycoprotein ZP3; zona pellucida glycoprotein 3A (sperm receptor); zona pellucida glycoprotein 3 (sperm receptor); Sperm receptor; ZP3A/ZP3B; Zona pellucida glycoprotein 3; Zp-3; Zona pellucida protein CCleaved into the following chain:Processed zona pellucida sperm-binding protein 3

ZP3

ZP3; ZP3; ZPC; ZP3A; ZP3B; Zp-3; ZP3A; ZP3B; ZPC; Zp-3

ZP3_HUMAN

Human

424

This assay has high sensitivity and excellent specificity for detection of human ZP3. No significant cross-reactivity or interference between human ZP3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human ZP3 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ZP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

160358850

NP_001103824.1

NM_001110354.1

P21754

47,018 Da

Ovarian Infertility Genes Pathway (198801)

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]

ZP3: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. Belongs to the ZP domain family. ZPC subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Extracellular matrix. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; acrosome; extracellular region; integral to membrane; secretory granule; multivesicular body; extracellular matrix; proteinaceous extracellular matrix; perinuclear region of cytoplasm; cytoplasm; plasma membrane. Molecular Function: acrosin binding; store-operated calcium channel activity; signal transducer activity; protein binding; carbohydrate binding; manganese ion transmembrane transporter activity. Biological Process: positive regulation of type IV hypersensitivity; phosphoinositide-mediated signaling; binding of sperm to zona pellucida; positive regulation of transcription, DNA-dependent; positive regulation of leukocyte migration; manganese ion transport; blastocyst formation; positive regulation of phosphatidylinositol biosynthetic process; multicellular organism reproduction; positive regulation of interleukin-4 production; positive regulation of protein kinase B signaling cascade; humoral immune response mediated by circulating immunoglobulin; intracellular protein transport; positive regulation of interferon-gamma production; positive regulation of humoral immune response; positive regulation of protein kinase activity; single fertilization; positive regulation of T cell proliferation; negative regulation of transcription, DNA-dependent; oocyte development; positive regulation of inflammatory response

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800