catalog number :
MBS945610
products type :
ELISA Kit
products full name :
Dog motilin, MLN ELISA Kit
products short name :
motilin
products name syn :
Dog motilin (MLN) ELISA kit; MGC138519; ; motilin
other names :
Motilin; Motilin; motilin
other gene names :
MLN; MLN
uniprot entry name :
MOTI_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of dog MLN. No significant cross-reactivity or interference between dog MLN and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 125 pg/ml-8000 pg/ml. Sensitivity: Typically less than 31.25 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
2,685 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle.
size4 :
10x96-Strip-Wells