Human Melanocyte protein PMEL, SILV ELISA Kit | MyBioSource MBS945451 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Melanocyte protein PMEL, SILV ELISA Kit

catalog :

MBS945451

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS945451

products type :

ELISA Kit

products full name :

Human Melanocyte protein PMEL, SILV ELISA Kit

products short name :

silver homolog (mouse)

products name syn :

Human Melanocyte protein PMEL (SILV) ELISA kit; D12S53E; ME20; PMEL; PMEL17; SI; SIL; gp100; Melanocyte protein mel 17; Pmel 17; Silver; mouse; homolog of; melanosomal matrix protein17; silver (mouse homolog) -like; silver homolog; silver homolog (mouse)

other names :

melanocyte protein PMEL isoform 2; Melanocyte protein PMEL; melanocyte protein PMEL; melanocyte protein mel 17; silver, mouse, homolog of; melanocyte protein Pmel 17; melanosomal matrix protein17; silver locus protein homolog; melanoma-associated ME20 antigen; melanocytes lineage-specific antigen GP100; premelanosome protein; ME20-M; ME20M; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; P1; P100; Premelanosome protein; Silver locus protein homologCleaved into the following 2 chains:M-alphaAlternative name(s):95 kDa melanocyte-specific secreted glycoprotein; P26; Secreted melanoma-associated ME20 antigen; ME20-S; ME20S

products gene name :

SILV

other gene names :

PMEL; PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E; D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S

uniprot entry name :

PMEL_HUMAN

reactivity :

Human

sequence length :

575

specificity :

This assay has high sensitivity and excellent specificity for detection of Human SILV. No significant cross-reactivity or interference between Human SILV and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: 5.86 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: biogenic amine biosynthesis and metabolism. Protein Biological Process 3: Melanin biosynthesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SILV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SILV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SILV is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SILV bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

318037593

ncbi acc num :

NP_001186982.1

ncbi gb acc num :

NM_001200053.1

uniprot acc num :

P40967

ncbi mol weight :

70,255 Da

ncbi summary :

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

uniprot summary :

SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Vesicle. Chromosomal Location of Human Ortholog: 12q13-q14. Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; extracellular region; melanosome; integral to membrane; plasma membrane. Molecular Function: protein binding. Biological Process: melanin biosynthetic process; melanosome organization and biogenesis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage