catalog number :
MBS945444
products type :
ELISA Kit
products full name :
Rat thyrotropin-releasing hormone, TRH ELISA Kit
products short name :
thyrotropin-releasing hormone
products name syn :
Rat thyrotropin-releasing hormone; TRH ELISA KIT; MGC125964; MGC125965; ; thyrotropin-releasing hormone
other names :
thyrotropin releasing hormone preproprotein; Pro-thyrotropin-releasing hormone; thyrotropin releasing hormone; protirelin; prothyroliberin; TSH-releasing factor; thyrotropin-releasing factor; pro-thyrotropin-releasing hormone; thyrotropin releasing hormone; ProthyroliberinCleaved into the following chain:Thyrotropin-releasing hormone; TRHAlternative name(s):Protirelin; TSH-releasing factor; Thyroliberin; Thyrotropin-releasing factor; TRF
other gene names :
Trh; Trh; THR; Trf; TRH01; Pro-TRH; Pro-TRH; TRH; TRF
uniprot entry name :
TRH_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat TRH. No significant cross-reactivity or interference between Rat TRH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.5 pg/ml-100 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.25 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for TRH and Horseradish Peroxidase (HRP) conjugated TRH. The competitive inhibition reaction is launched between with HRP labeled TRH and unlabeled TRH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TRH in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_037178.1
ncbi gb acc num :
NM_013046.3
ncbi mol weight :
29,284 Da
ncbi pathways :
G Alpha (q) Signalling Events Pathway 574254!!GPCR Downstream Signaling Pathway 574250!!Signal Transduction Pathway 574116!!Signaling By GPCR Pathway 574222
ncbi summary :
This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The rat proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin, and may be involved in cardiac function in heart failure. Disruption of this gene is associated with hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, May 2013]
uniprot summary :
Function: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.