Human Signal-induced proliferation-associated protein 1, SIPA1 ELISA Kit | MyBioSource MBS945430 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Signal-induced proliferation-associated protein 1, SIPA1 ELISA Kit

catalog :

MBS945430

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS945430

products type :

ELISA Kit

products full name :

Human Signal-induced proliferation-associated protein 1, SIPA1 ELISA Kit

products short name :

signal-induced proliferation-associated 1

products name syn :

Human Signal-induced proliferation-associated protein 1 (SIPA1) ELISA kit; MGC102688; MGC17037; SPA1; GTPase-activating protein Spa-1; p130 SPA-1; signal-induced proliferation-associated gene 1; signal-induced proliferation-associated protein 1; signal-induced proliferation-associated 1

other names :

signal-induced proliferation-associated protein 1; Signal-induced proliferation-associated protein 1; signal-induced proliferation-associated protein 1; sipa-1; p130 SPA-1; GTPase-activating protein Spa-1; signal-induced proliferation-associated gene 1; signal-induced proliferation-associated 1; GTPase-activating protein Spa-1; p130 SPA-1

products gene name :

SIPA1

other gene names :

SIPA1; SIPA1; SPA1; SPA1; Sipa-1

uniprot entry name :

SIPA1_HUMAN

reactivity :

Human

sequence length :

1042

specificity :

This assay has high sensitivity and excellent specificity for detection of human SIPA1. No significant cross-reactivity or interference between human SIPA1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human SIPA1 is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIPA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIPA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIPA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIPA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

206725523

ncbi acc num :

NP_006738.3

ncbi gb acc num :

NM_006747.3

uniprot acc num :

Q96FS4

ncbi mol weight :

112,149 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Immune System Pathway (106386); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); MAPK Cascade Pathway (198834); Rap1 Signalling Pathway (187108)

ncbi summary :

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]

uniprot summary :

SIPA1: GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state. Protein type: GAPs, Ras; GAPs. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: Golgi apparatus; transport vesicle; protein complex; perinuclear region of cytoplasm; nucleolus; plasma membrane; nucleus; cytosol. Molecular Function: protein C-terminus binding; protein binding; GTPase activator activity. Biological Process: cell proliferation; cellular response to water deprivation; cytoskeleton organization and biogenesis; negative regulation of cell growth; negative regulation of cell adhesion; signal transduction; negative regulation of cell cycle; positive regulation of GTPase activity

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage