ELISA/assay

Human Mammaglobin-B, SCGB2A1 ELISA Kit

MBS945304

48-Strip-Wells

510 USD

ELISA Kit

Human Mammaglobin-B, SCGB2A1 ELISA Kit

secretoglobin, family 2A, member 1

Human Mammaglobin-B (SCGB2A1) ELISA kit; LPHC; MGB2; MGC71973; UGB3; lacryglobin; lipophilin C; mammaglobin 2; mammaglobin B; secretoglobin; family 2A; member 1

mammaglobin-B; Mammaglobin-B; mammaglobin-B; lacryglobin; lipophilin C; lipophilin-C; mammaglobin 2; mammaglobin B; mammaglobin-2; secretoglobin, family 2A, member 1; Lacryglobin; Lipophilin-C; Mammaglobin-2; Secretoglobin family 2A member 1

SCGB2A1

SCGB2A1; SCGB2A1; LPHC; LPNC; MGB2; UGB3; LIPHC; MGB2; UGB3

SG2A1_HUMAN

Human

95

This assay has high sensitivity and excellent specificity for detection of human SCGB2A1. No significant cross-reactivity or interference between human SCGB2A1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.235 ng/ml -15 ng/ml. Sensitivity: The minimum detectable dose of human SCGB2A1 is typically less than 0.058 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SCGB2A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCGB2A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCGB2A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCGB2A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4505171

NP_002398.1

NM_002407.2

O75556

10,884 Da

SCGB2A1: May bind androgens and other steroids, may also bind estramustine, a chemotherapeutic agent used for prostate cancer. May be under transcriptional regulation of steroid hormones. Belongs to the secretoglobin family. Lipophilin subfamily. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space. Molecular Function: protein heterodimerization activity. Biological Process: androgen receptor signaling pathway

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800