ELISA/assay

Dog arginine vasopressin, AVP ELISA Kit

MBS945283

48-Strip-Wells

565 USD

ELISA Kit

Dog arginine vasopressin, AVP ELISA Kit

arginine vasopressin (AVP)

Dog arginine vasopressin (AVP) ELISA kit; arginine vasopressin (AVP)

vasopressin-neurophysin 2-copeptin preproprotein; Vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin 2-copeptin; neurohypophyseal; prepro-AVP-NP II; antidiuretic hormone; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; arginine vasopressin; AVP-NPIICleaved into the following 3 chains:Arg-vasopressinAlternative name(s):Arginine-vasopressin

AVP

AVP; AVP; VP; ADH; ARVP; AVRP; AVP-NPII; ARVP; VP

NEU2_HUMAN

Dog

164

This assay has high sensitivity and excellent specificity for detection of dog AVP. No significant cross-reactivity or interference between dog AVP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.7 8 pg/ml-50 pg/ml. Sensitivity: The minimum detectable dose of dog AVP is typically less than 0.2 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AVP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AVP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AVP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AVP bound in the initial step. The color development is stopped and the intensity of the color is measured.

13259533

NP_000481.2

NM_000490.4

P01185

17,325 Da

Aquaporin-mediated Transport Pathway (187187); BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway (477138); Circadian Clock Pathway (187173); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358); Regulation Of Water Balance By Renal Aquaporins Pathway (187189)

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]

AVP: Neurophysin 2 specifically binds vasopressin. Defects in AVP are the cause of diabetes insipidus, neurohypophyseal (NDI). A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopression deficiency during childhood. Belongs to the vasopressin/oxytocin family. Protein type: Secreted, signal peptide; Secreted; Hormone. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: extracellular space; dendrite; extracellular region; cytosol; secretory granule. Molecular Function: neuropeptide hormone activity; signal transducer activity; V1A vasopressin receptor binding; caspase inhibitor activity; neurohypophyseal hormone activity; V1B vasopressin receptor binding; protein kinase activity; receptor binding. Biological Process: response to nicotine; circadian rhythm; water transport; positive regulation of cellular pH reduction; positive regulation of glutamate secretion; locomotory behavior; positive regulation of systemic arterial blood pressure; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; elevation of cytosolic calcium ion concentration; hyperosmotic salinity response; cell-cell signaling; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; transmembrane transport; positive regulation of prostaglandin biosynthetic process; penile erection; grooming behavior; vasoconstriction; sodium-independent organic anion transport; generation of precursor metabolites and energy; maternal behavior; social behavior; response to testosterone stimulus; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; negative regulation of female receptivity; response to ethanol; negative regulation of transmission of nerve impulse; positive regulation of vasoconstriction; renal water homeostasis; negative regulation of apoptosis. Disease: Diabetes Insipidus, Neurohypophyseal

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150