Human Bromodomain-containing protein 4, BRD4 ELISA Kit | MyBioSource MBS945281 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Bromodomain-containing protein 4, BRD4 ELISA Kit

catalog :

MBS945281

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS945281

products type :

ELISA Kit

products full name :

Human Bromodomain-containing protein 4, BRD4 ELISA Kit

products short name :

bromodomain containing 4

products name syn :

Human Bromodomain-containing protein 4 (BRD4) ELISA kit; CAP; HUNK1; HUNKI; MCAP; bromodomain-containing 4; bromodomain-containing protein 4; chromosome-associated protein; bromodomain containing 4

other names :

bromodomain-containing protein 4 isoform short; Bromodomain-containing protein 4; bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein; bromodomain containing 4; Protein HUNK1

products gene name :

BRD4

other gene names :

BRD4; BRD4; CAP; MCAP; HUNK1; HUNKI; HUNK1

uniprot entry name :

BRD4_HUMAN

reactivity :

Human

sequence length :

722

specificity :

This assay has high sensitivity and excellent specificity for detection of human BRD4. No significant cross-reactivity or interference between human BRD4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of human BRD4 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BRD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BRD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BRD4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BRD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

7657218

ncbi acc num :

NP_055114.1

ncbi gb acc num :

NM_014299.2

uniprot acc num :

O60885

ncbi mol weight :

152,219 Da

ncbi summary :

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

BRD4: an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described. Protein type: Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BRD family. Chromosomal Location of Human Ortholog: 19p13.1. Cellular Component: nucleoplasm; transcription elongation factor complex b; condensed nuclear chromosome; nuclear chromatin; cytoplasm; chromosome; nucleus. Molecular Function: protein binding; DNA binding; p53 binding; chromatin binding. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; transcription, DNA-dependent; inner cell mass cell proliferation; protein amino acid phosphorylation; chromosome segregation; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; positive regulation of RNA elongation from RNA polymerase II promoter; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of DNA binding

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage