ELISA/assay

Human Mimecan, OGN ELISA Kit

MBS945279

48-Strip-Wells

510 USD

ELISA Kit

Human Mimecan, OGN ELISA Kit

osteoglycin

Human Mimecan (OGN) ELISA kit; DKFZp586P2421; OG; OIF; SLRR3A; corneal keratan sulfate proteoglycan; mimecan proteoglycan; osteoinductive factor; osteoglycin

mimecan preproprotein; Mimecan; mimecan; mimecan proteoglycan; osteoinductive factor; corneal keratan sulfate proteoglycan; osteoglycin; Osteoglycin; Osteoinductive factor; OIF

OGN

OGN; OGN; OG; OIF; SLRR3A; OIF; SLRR3A; OIF

MIME_HUMAN

Human

298

This assay has high sensitivity and excellent specificity for detection of human OGN. No significant cross-reactivity or interference between human OGN and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human OGN is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OGN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OGN bound in the initial step. The color development is stopped and the intensity of the color is measured.

7661704

NP_054776.1

NM_014057.3

P20774

33,922 Da

Glycosaminoglycan Metabolism Pathway (645297); Keratan Sulfate Biosynthesis Pathway (645302); Keratan Sulfate Degradation Pathway (645303); Keratan Sulfate/keratin Metabolism Pathway (645301); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196)

This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). Alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

OGN: Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q22. Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; proteinaceous extracellular matrix; Golgi lumen; extracellular region; vesicle. Molecular Function: protein binding; growth factor activity. Biological Process: keratan sulfate metabolic process; glycosaminoglycan metabolic process; negative regulation of smooth muscle cell proliferation; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; keratan sulfate catabolic process

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800