ELISA/assay

Mouse Tumor necrosis factor ligand superfamily member 14, TNFSF14 ELISA Kit

MBS945084

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Tumor necrosis factor ligand superfamily member 14, TNFSF14 ELISA Kit

[tumor necrosis factor (ligand) superfamily, member 14]

[Mouse Tumor necrosis factor ligand superfamily member 14 (TNFSF14) ELISA kit; CD258; HVEML; LIGHT; LTg; TR2; delta transmembrane LIGHT; herpesvirus entry mediator A; ligand for herpesvirus entry mediator; tumor necrosis factor ligand superfamily; member 14; tumor necrosis factor; tumor necrosis factor (ligand) superfamily; member 14]

[tumor necrosis factor ligand superfamily member 14; Tumor necrosis factor ligand superfamily member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor (ligand) superfamily, member 14; CD_antigen: CD258Cleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form]

[TNFSF14]

[Tnfsf14; Tnfsf14; LTg; HVEML; LIGHT; Ly113; HVEM-L; Light]

TNF14_MOUSE

Mouse

239

This assay has high sensitivity and excellent specificity for detection of human TNFAIP6. No significant cross-reactivity or interference between human TNFAIP6 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, Plasma, Cell Culture Supernates. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNFAIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFAIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

9507195

NP_062291.1

NM_019418.3

Q9QYH9

26,338 Da

Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865); NF-kappa B Signaling Pathway (634537)

TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Inhibitor; Cytokine; Membrane protein, integral. Cellular Component: extracellular space; membrane; extracellular region; plasma membrane; integral to membrane. Molecular Function: caspase inhibitor activity; cytokine activity; tumor necrosis factor receptor binding; receptor binding. Biological Process: release of cytoplasmic sequestered NF-kappaB; T cell costimulation; immune response; negative regulation of caspase activity; positive regulation of myoblast differentiation

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145