product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Neudesin, NENF ELISA Kit
catalog :
MBS945077
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS945077
products type :
ELISA Kit
products full name :
Mouse Neudesin, NENF ELISA Kit
products short name :
neuron derived neurotrophic factor
products name syn :
Mouse Neudesin (NENF) ELISA kit; CIR2; NEUDESIN; SCIRP10; SPUF; SCIRP10-related protein; Spinal cord injury related protein 10; cell growth-inhibiting protein 47; cell immortalization-related; secreted protein of unknown function; neuron derived neurotrophic factor
other names :
neudesin; Neudesin; neudesin; SCIRP10-related protein; neuron-derived neurotrophic factor; secreted protein of unknown function; Spinal cord injury related protein 10; neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; Secreted protein of unknown function; SPUF protein
products gene name :
NENF
other gene names :
Nenf; Nenf; Spuf; SCIRP10; 1110060M21Rik; Spuf; SPUF protein
uniprot entry name :
NENF_MOUSE
reactivity :
Mouse
sequence length :
171
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse NENF. No significant cross-reactivity or interference between Mouse NENF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NENF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NENF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NENF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NENF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
13384818
ncbi acc num :
NP_079700.1
ncbi gb acc num :
NM_025424.2
uniprot acc num :
Q9CQ45
ncbi mol weight :
18,904 Da
uniprot summary :
NENF: Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme. Belongs to the cytochrome b5 family. MAPR subfamily. Protein type: Secreted, signal peptide; Secreted. Cellular Component: nucleoplasm; extracellular space; membrane; extracellular region; endomembrane system; nucleus. Molecular Function: growth factor activity; metal ion binding; heme binding. Biological Process: positive regulation of MAPKKK cascade; negative regulation of appetite
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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