product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Tryptase beta-2, TPSB2 ELISA Kit
catalog :
MBS945041
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS945041
products type :
ELISA Kit
products full name :
Mouse Tryptase beta-2, TPSB2 ELISA Kit
products short name :
tryptase beta 2
products name syn :
Mouse Tryptase beta-2 (TPSB2) ELISA kit; TPS2; tryptaseB; tryptaseC; OTTHUMP00000208944; mast cell tryptase beta II; mast cell tryptase beta III; tryptase II; tryptase III; tryptase beta 2; tryptase beta II; tryptase beta III; tryptase beta 2
other names :
Tryptase beta-2; Tryptase beta-2; Mast cell protease 6; mMCP-6
products gene name :
TPSB2
other gene names :
Tpsb2; Mcpt6; Tryptase-2; mMCP-6
uniprot entry name :
TRYB2_MOUSE
reactivity :
Mouse
sequence length :
276
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse TPSB2. No significant cross-reactivity or interference between mouse TPSB2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml -5000 pg/ml. Sensitivity: The minimum detectable dose of mouse TPSB2 is typically less than 19.5 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPSB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPSB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPSB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPSB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
126839
ncbi acc num :
P21845.2
uniprot acc num :
P21845
ncbi mol weight :
30,927 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
TPSB2: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Belongs to the peptidase S1 family. Tryptase subfamily. Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.59. Cellular Component: extracellular matrix; extracellular space; extracellular region. Molecular Function: peptidase activity; heparin binding; protein binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity. Biological Process: proteolysis; inflammatory response
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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