Human Interleukin-36 beta, IL1F8 ELISA Kit | MyBioSource MBS944849 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interleukin-36 beta, IL1F8 ELISA Kit

catalog :

MBS944849

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS944849

products type :

ELISA Kit

products full name :

Human Interleukin-36 beta, IL1F8 ELISA Kit

products short name :

interleukin 1 family, member 8 (eta)

products name syn :

Human Interleukin-36 beta (IL1F8) ELISA kit; FIL1; FIL1- (ETA); FIL1H; IL-1F8; IL-1H2; IL1-ETA; IL1H2; MGC126880; MGC126882; IL-1F8 (FIL1-eta) ; IL1F8 (Canonical product IL-1F8a) ; Interleukin-1 Superfamily e; family of interleukin 1-eta; interleukin; interleukin 1 family; member 8 (eta)

other names :

interleukin-36 beta isoform 1; Interleukin-36 beta; interleukin-36 beta; FIL1 eta; IL-1 eta; IL-1F8 (FIL1-eta); interleukin-1 eta; interleukin 1, eta; interleukin-1 homolog 2; Interleukin-1 Superfamily e; family of interleukin 1-eta; interleukin-1 family member 8; IL1F8 (Canonical product IL-1F8a); interleukin 1 family, member 8 (eta); interleukin 36, beta; FIL1 eta; Interleukin-1 eta; IL-1 eta; Interleukin-1 family member 8; IL-1F8; Interleukin-1 homolog 2; IL-1H2

products gene name :

IL1F8

other gene names :

IL36B; IL36B; FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA); IL1F8; IL1H2; IL-1 eta; IL-1F8; IL-1H2

uniprot entry name :

IL36B_HUMAN

reactivity :

Human

sequence length :

164

specificity :

This assay has high sensitivity and excellent specificity for detection of human IL1F8. No significant cross-reactivity or interference between human IL1F8 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human IL1F8 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1F8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1F8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1F8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1F8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

20070252

ncbi acc num :

NP_055253.2

ncbi gb acc num :

NM_014438.4

uniprot acc num :

Q9NZH7

ncbi mol weight :

18,522 Da

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

uniprot summary :

IL36B: Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region. Molecular Function: interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-6 production; innate immune response; immune response; inflammatory response

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage