catalog number :
MBS944814
products type :
ELISA Kit
products full name :
Rat Secretogranin-3, SCG3 ELISA Kit
products short name :
secretogranin III
products name syn :
Rat Secretogranin-3 (SCG3) ELISA kit; FLJ90833; SGIII; ; secretogranin III
other names :
secretogranin-3; Secretogranin-3; secretogranin-3; 1B1075; secretogranin III; 1B1075; Secretogranin III; SgIII
products gene name :
SCG3
other gene names :
Scg3; Scg3; SgIII; SgIII
uniprot entry name :
SCG3_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat SCG3. No significant cross-reactivity or interference between rat SCG3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of rat SCG3 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SCG3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCG3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCG3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_446308.1
ncbi gb acc num :
NM_053856.2
ncbi mol weight :
53,183 Da
ncbi pathways :
Hemostasis Pathway (648959); Platelet Activation, Signaling And Aggregation Pathway (648969); Platelet Degranulation Pathway (648981); Response To Elevated Platelet Cytosolic Ca2+ Pathway (648979)
ncbi summary :
component of intracellular dense core vesicles [RGD, Feb 2006]
uniprot summary :
SCG3: 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region; cytoplasmic vesicle. Molecular Function: protein binding
size4 :
10x96-Strip-Wells