ELISA/assay

Human Interferon-induced GTP-binding protein Mx1, MX1 ELISA Kit

MBS944720

48-Strip-Wells

510 USD

ELISA Kit

Human Interferon-induced GTP-binding protein Mx1, MX1 ELISA Kit

myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse)

Human Interferon-induced GTP-binding protein Mx1 (MX1) ELISA kit; IFI-78K; IFI78; MX; MxA; interferon inducible protein p78; interferon-induced GTP-binding protein Mx1; interferon-regulated resistance GTP-binding protein; myxovirus resistance protein 1; myxovirus (influenza virus) resistance 1; interferon-inducible protein p78 (mouse)

interferon-induced GTP-binding protein Mx1; Interferon-induced GTP-binding protein Mx1; interferon-induced GTP-binding protein Mx1; myxoma resistance protein 1; myxovirus resistance protein 1; interferon-inducible protein p78; interferon-inducible myxovirus resistance-1 protein; myxovirus (influenza) resistance 1, (murine homolog); myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse); Myxoma resistance protein 1; Myxovirus resistance protein 1Cleaved into the following chain:Interferon-induced GTP-binding protein Mx1, N-terminally processed

MX1

MX1; MX1; Mx1B; Mx1-b

MX1_BOVIN

Human

648

This assay has high sensitivity and excellent specificity for detection of human MX1. No significant cross-reactivity or interference between human MX1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human MX1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

27806841

NP_776365.1

NM_173940.2

P79135

74,805 Da

Influenza A Pathway (217172); Influenza A Pathway (217150); Measles Pathway (213304); Measles Pathway (213277)

Function: Interferon-induced dynamin-like GTPase with antiviral activity against rabies virus (RABV), vesicular stomatitis virus (VSV) and murine pneumonia virus (MPV). Isoform 1 but not isoform 2 shows antiviral activity against vesicular stomatitis virus (VSV).3 PublicationsManual assertion based on experiment in:Ref.5

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800