Human Tropomyosin alpha-4 chain, TPM4 ELISA Kit | MyBioSource MBS944560 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tropomyosin alpha-4 chain, TPM4 ELISA Kit

catalog :

MBS944560

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS944560

products type :

ELISA Kit

products full name :

Human Tropomyosin alpha-4 chain, TPM4 ELISA Kit

products short name :

[tropomyosin 4]

products name syn :

[Human Tropomyosin alpha-4 chain (TPM4) ELISA kit; tropomyosin 4]

other names :

[tropomyosin alpha-4 chain isoform 1; Tropomyosin alpha-4 chain; tropomyosin alpha-4 chain; TM30p1; tropomyosin-4; epididymis secretory protein Li 108; tropomyosin 4; TM30p1; Tropomyosin-4]

products gene name :

[TPM4]

other gene names :

[TPM4; TPM4; HEL-S-108]

uniprot entry name :

TPM4_HUMAN

reactivity :

Human

sequence length :

248

specificity :

This assay has high sensitivity and excellent specificity for detection of human TPI1. No significant cross-reactivity or interference between human TPI1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 28 pg/ml-1800 pg/ml. Sensitivity: 7 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPI1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

223555975

ncbi acc num :

NP_001138632.1

ncbi gb acc num :

NM_001145160.1

uniprot acc num :

P67936

ncbi mol weight :

28,522 Da

ncbi pathways :

Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261); Smooth Muscle Contraction Pathway (161025); Striated Muscle Contraction Pathway (106262); Striated Muscle Contraction Pathway (198903)

ncbi summary :

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

uniprot summary :

TPM4: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In nonmuscle cells is implicated in stabilizing cytoskeleton actin filaments and endocytosis. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19p13.1. Cellular Component: cortical cytoskeleton; filamentous actin; cytoskeleton; focal adhesion; membrane; stress fiber; podosome; cytosol; muscle thin filament tropomyosin. Molecular Function: structural constituent of muscle; calcium ion binding; actin binding. Biological Process: osteoblast differentiation; muscle contraction; cell motility; muscle filament sliding

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145

more info or order :

MyBioSource product webpage