ELISA/assay

Human Galectin-2, LGALS2 ELISA Kit

MBS944521

48-Strip-Wells

510 USD

ELISA Kit

Human Galectin-2, LGALS2 ELISA Kit

lectin, galactoside-binding, soluble, 2

Human Galectin-2 (LGALS2) ELISA kit; HL14; MGC75071; S-Lac lectin 2; beta-galactoside-binding lectin L-14-II; galectin 2; lactose-binding lectin 2; lectin; galactoside-binding; soluble; 2

galectin-2; Galectin-2; galectin-2; gal-2; galectin 2; S-Lac lectin 2; lactose-binding lectin 2; beta-galactoside-binding lectin L-14-II; lectin, galactoside-binding, soluble, 2; Beta-galactoside-binding lectin L-14-II; HL14; Lactose-binding lectin 2; S-Lac lectin 2

LGALS2

LGALS2; LGALS2; HL14; Gal-2

LEG2_HUMAN

Human

132

This assay has high sensitivity and excellent specificity for detection of human galectin-2. No significant cross-reactivity or interference between human galectin-2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml -80 ng/ml. Sensitivity: The minimum detectable dose of human galectin-2 is typically less than 0.312 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for galectin-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any galectin-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for galectin-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of galectin-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

5729903

NP_006489.1

NM_006498.2

P05162

14,644 Da

The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. A single nucleotide polymorphism in an intron of this gene can alter the transcriptional level of the protein, with a resultant increased risk of myocardial infarction. [provided by RefSeq, Jul 2008]

LGALS2: This protein binds beta-galactoside. Its physiological function is not yet known. Chromosomal Location of Human Ortholog: 22q13.1. Molecular Function: galactoside binding; carbohydrate binding. Disease: Myocardial Infarction, Susceptibility To

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800