ELISA/assay

Human Low affinity immunoglobulin gamma Fc region receptor III-A, FCGR3A ELISA Kit

MBS944475

48-Strip-Wells

510 USD

ELISA Kit

Human Low affinity immunoglobulin gamma Fc region receptor III-A, FCGR3A ELISA Kit

Fc fragment of IgG, low affinity IIIa, receptor (CD16a)

Human Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A) ELISA kit; RP11-5K23.1; CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; Fc fragment of IgG; low affinity III; receptor for (CD16) ; Fc gamma receptor III-A; Fc-gamma RIII-alpha; Fc-gamma RIIIa; F; Fc fragment of IgG; low affinity IIIa; receptor (CD16a)

low affinity immunoglobulin gamma Fc region receptor III-A isoform a; Low affinity immunoglobulin gamma Fc region receptor III-A; low affinity immunoglobulin gamma Fc region receptor III-A; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-gamma receptor III-2 (CD 16); immunoglobulin G Fc receptor III; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: CD16a

FCGR3A

FCGR3A; FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA; CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa

FCG3A_HUMAN

Human

254

This assay has high sensitivity and excellent specificity for detection of human FCGR3A. No significant cross reactivity or interference between human FCGR3A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Detection Range: 47 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of human FCGR3A is typically less than 11.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FCGR3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCGR3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCGR3A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCGR3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

50726979

NP_000560.5

NM_000569.6

P08637

29,089 Da

Adaptive Immune System Pathway (366160); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Leishmaniasis Pathway (144181); Leishmaniasis Pathway (144028); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490); Osteoclast Differentiation Pathway (193147)

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23. Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: IgG binding. Biological Process: regulation of immune response; innate immune response; immune response. Disease: Immunodeficiency 20

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800