catalog number :
MBS944418
products type :
ELISA Kit
products full name :
Dog Interleukin-2 receptor subunit alpha, IL2RA ELISA Kit
products short name :
interleukin 2 receptor, alpha
products name syn :
Dog Interleukin-2 receptor subunit alpha (IL2RA) ELISA kit; RP11-536K7.1; CD25; IDDM10; IL2R; TCGFR; ; interleukin 2 receptor; alpha
other names :
interleukin-2 receptor subunit alpha; Interleukin-2 receptor subunit alpha; interleukin-2 receptor subunit alpha; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; interleukin 2 receptor alpha chain; interleukin-2 receptor alpha subunit; TAC antigen; p55; CD_antigen: CD25
products gene name :
IL2RA
other gene names :
IL2RA; IL2RA; CD25; IL-2RA; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
uniprot entry name :
IL2RA_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of dog IL2RA. No significant cross-reactivity or interference between dog IL2RA and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of dog interleukin-2 receptor subunit alpha (IL2RA) concentrations in serum, plasma, tissue homogenates. Detection Range: 62.5 pg/ml - 4000 pg/ml.
other info2 :
Sensitivity: The minimum detectable dose of dog IL2RA is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL2RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL2RA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL2RA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL2RA bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001003211.1
ncbi gb acc num :
NM_001003211.1
ncbi mol weight :
30,357 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway 581155!!Cytokine-cytokine Receptor Interaction Pathway 84101!!Cytokine-cytokine Receptor Interaction Pathway 460!!Endocytosis Pathway 102295!!Endocytosis Pathway 102181!!HTLV-I Infection Pathway 373907!!HTLV-I Infection Pathway 373889!!Hematopoietic Cell Lineage Pathway 84128!!Hematopoietic Cell Lineage Pathway 489!!IL-2 Signaling Pathway 198596
uniprot summary :
Function: Receptor for interleukin-2.