Human Protein-glutamine gamma-glutamyltransferase K, TGM1 ELISA Kit | MyBioSource MBS944411 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein-glutamine gamma-glutamyltransferase K, TGM1 ELISA Kit

catalog :

MBS944411

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS944411

products type :

ELISA Kit

products full name :

Human Protein-glutamine gamma-glutamyltransferase K, TGM1 ELISA Kit

products short name :

transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)

products name syn :

Human Protein-glutamine gamma-glutamyltransferase K (TGM1) ELISA kit; ICR2; KTG; LI; LI1; TGASE; TGK; TGase K; epidermal TGase; transglutaminase 1; transglutaminase K; transglutaminase; keratinocyte; transglutaminase 1 (K polypeptide epidermal type I; protein-glutamine-gamma-glutamyltransferase)

other names :

protein-glutamine gamma-glutamyltransferase K; Protein-glutamine gamma-glutamyltransferase K; protein-glutamine gamma-glutamyltransferase K; TG(K); TGase K; TGase-1; epidermal TGase; transglutaminase K; transglutaminase-1; transglutaminase 1 isoform; transglutaminase, keratinocyte; K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase; transglutaminase 1; Epidermal TGase; Transglutaminase K; TG(K); TGK; TGase K; Transglutaminase-1; TGase-1

products gene name :

TGM1

other gene names :

TGM1; TGM1; LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE; KTG; TG(K); TGK; TGase K; TGase-1

uniprot entry name :

TGM1_HUMAN

reactivity :

Human

sequence length :

817

specificity :

This assay has high sensitivity and excellent specificity for detection of Human TGM1. No significant cross-reactivity or interference between Human TGM1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml-1600 pg/ml. Sensitivity: 6.25 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Keratinization

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507475

ncbi acc num :

NP_000350.1

ncbi gb acc num :

NM_000359.2

uniprot acc num :

P22735

ncbi mol weight :

89,787 Da

ncbi summary :

The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE). [provided by RefSeq, Jul 2008]

uniprot summary :

TGM1: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Protein type: Transferase; EC 2.3.2.13. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: cell-cell adherens junction; cornified envelope; intrinsic to membrane. Molecular Function: protein binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding. Biological Process: keratinocyte differentiation; cell envelope organization and biogenesis; organ morphogenesis; keratinization; protein modification process; peptide cross-linking. Disease: Ichthyosis, Congenital, Autosomal Recessive 1

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage