catalog number :
MBS944301
products type :
ELISA Kit
products full name :
Bovine fibroblast growth factor 21, FGF21 ELISA Kit
products short name :
[fibroblast growth factor 21]
products name syn :
[Bovine fibroblast growth factor 21 (FGF21) ELISA kit; fibroblast growth factor 21]
other names :
[fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; fibroblast growth factor 21]
products gene name :
[FGF21]
other gene names :
[FGF21; FGF21; UNQ3115/PRO10196; FGF-21]
uniprot entry name :
FGF21_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine FGF21. No significant cross-reactivity or interference between Bovine FGF21 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF21 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_061986.1
ncbi gb acc num :
NM_019113.2
ncbi mol weight :
22,300 Da
ncbi pathways :
MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Melanoma Pathway (83114); Melanoma Pathway (526); Pathways In Cancer (83105); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500)
ncbi summary :
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]
uniprot summary :
FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: extracellular region. Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding. Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells