Mouse Annexin A1, ANXA1 ELISA Kit | MyBioSource MBS944251 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Annexin A1, ANXA1 ELISA Kit

catalog :

MBS944251

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS944251

products type :

ELISA Kit

products full name :

Mouse Annexin A1, ANXA1 ELISA Kit

products short name :

annexin A1

products name syn :

Mouse Annexin A1 (ANXA1) ELISA kit; ANX1; LPC1; OTTHUMP00000021475; annexin I; annexin I (lipocortin I) ; lipocortin I; annexin A1

other names :

annexin A1; Annexin A1; annexin A1; p35; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin 1; lipocortin I; chromobindin-9; phospholipase A2 inhibitory protein; annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

products gene name :

ANXA1

other gene names :

Anxa1; Anxa1; Lpc1; Anx-1; Lpc-1; Anx-A1; C430014K04Rik; Anx1; Lpc-1; Lpc1

uniprot entry name :

ANXA1_MOUSE

reactivity :

Mouse

sequence length :

346

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse ANXA1. No significant cross-reactivity or interference between mouse ANXA1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: Typically less than 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANXA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANXA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANXA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANXA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

124517663

ncbi acc num :

NP_034860.2

ncbi gb acc num :

NM_010730.2

uniprot acc num :

P10107

ncbi mol weight :

38,734 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (575426); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (575432); G Alpha (i) Signalling Events Pathway (575454); G Alpha (q) Signalling Events Pathway (575456); GPCR Downstream Signaling Pathway (575452); GPCR Ligand Binding Pathway (575425); Peptide Ligand-binding Receptors Pathway (575427); Prostaglandin Synthesis And Regulation Pathway (198286); Signal Transduction Pathway (575318); Signaling By GPCR Pathway (575424)

uniprot summary :

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion. Protein type: Lipid-binding; Calcium-binding. Cellular Component: extracellular space; focal adhesion; protein complex; mast cell granule; cell surface; cornified envelope; cilium; cell projection; membrane; cytoplasm; mitochondrial membrane; plasma membrane; nucleus; vesicle; endosome; sarcolemma. Molecular Function: protein binding, bridging; phospholipase inhibitor activity; protein binding; protein homodimerization activity; single-stranded RNA binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; calcium-dependent protein binding; helicase activity; single-stranded DNA binding. Biological Process: negative regulation of protein secretion; positive regulation of neutrophil apoptosis; positive regulation of apoptosis; alpha-beta T cell differentiation; positive regulation of vesicle fusion; neutrophil homeostasis; signal transduction; arachidonic acid secretion; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; positive regulation of prostaglandin biosynthetic process

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage