Human DNA damage-inducible transcript 4 protein, DDIT4 ELISA Kit | MyBioSource MBS944137 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human DNA damage-inducible transcript 4 protein, DDIT4 ELISA Kit

catalog :

MBS944137

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS944137

products type :

ELISA Kit

products full name :

Human DNA damage-inducible transcript 4 protein, DDIT4 ELISA Kit

products short name :

DNA-damage-inducible transcript 4

products name syn :

Human DNA damage-inducible transcript 4 protein (DDIT4) ELISA kit; Dig2; FLJ20500; REDD-1; REDD1; RP11-442H21.1; RTP801; HIF-1 responsive RTP801 (RTP801) ; RTP801; DNA-damage-inducible transcript 4

other names :

DNA damage-inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; protein regulated in development and DNA damage response 1; DNA-damage-inducible transcript 4; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1

products gene name :

DDIT4

other gene names :

DDIT4; DDIT4; Dig2; REDD1; REDD-1; REDD1; RTP801; REDD-1

uniprot entry name :

DDIT4_HUMAN

reactivity :

Human

sequence length :

232

specificity :

This assay has high sensitivity and excellent specificity for detection of human DDIT4. No significant cross-reactivity or interference between human DDIT4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, tissue homogenates, Cell lysates Cell lysates Cell lysates Cell lysates Cell lysates Cell lysates, urine, urine, urine, urine, urine, urine, cerebrospinal fluid (CSF). Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human DDIT4 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DDIT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DDIT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDIT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDIT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

9506687

ncbi acc num :

NP_061931.1

ncbi gb acc num :

NM_019058.2

uniprot acc num :

Q9NX09

ncbi mol weight :

25,371 Da

ncbi pathways :

Direct P53 Effectors Pathway (137939); MTOR Signaling Pathway (83059); MTOR Signaling Pathway (138001); MTOR Signaling Pathway (469)

uniprot summary :

DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family. Chromosomal Location of Human Ortholog: 10q22.1. Cellular Component: mitochondrion; cytoplasm; intracellular; cytosol. Biological Process: neuron differentiation; cell proliferation; protein complex disassembly; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; response to hypoxia; neuron migration; negative regulation of peptidyl-serine phosphorylation; brain development; negative regulation of glycolysis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage