ELISA/assay

Human Defensin-6, DEFA6 ELISA Kit

MBS944129

48-Strip-Wells

510 USD

ELISA Kit

Human Defensin-6, DEFA6 ELISA Kit

defensin, alpha 6, Paneth cell-specific

Human Defensin-6 (DEFA6) ELISA kit; DEF6; HD-6; defensin 6; defensin; alpha 6; defensin; alpha 6; Paneth cell-specific

defensin-6 preproprotein; Defensin-6; defensin-6; defensin 6; defensin, alpha 6, Paneth cell-specific; Defensin, alpha 6

DEFA6

DEFA6; DEFA6; DEF6; HD-6; DEF6

DEF6_HUMAN

Human

100

This assay has high sensitivity and excellent specificity for detection of human DEFA6. No significant cross-reactivity or interference between human DEFA6 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human DEFA6 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4503305

NP_001917.1

NM_001926.3

Q01524

10,975 Da

Alpha-defensins Pathway (530758); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 6, is highly expressed in the secretory granules of Paneth cells of the small intestine, and likely plays a role in host defense of human bowel. [provided by RefSeq, Jul 2008]

defensin, alpha 6: Has very low antimicrobial activity against Gram- negative and Gram-positive bacteria. May protect cells against infection with HIV-1. Belongs to the alpha-defensin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; Golgi lumen; extracellular region. Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800