ELISA/assay

Human Tyrosine-protein kinase ABL1, ABL1 ELISA Kit

MBS944092

48-Strip-Wells

510 USD

ELISA Kit

Human Tyrosine-protein kinase ABL1, ABL1 ELISA Kit

c-abl oncogene 1, receptor tyrosine kinase

Human Tyrosine-protein kinase ABL1 (ABL1) ELISA kit; RP11-83J21.1; ABL; JTK7; bcr/abl; c-ABL; p150; v-abl; bcr/c-abl oncogene protein; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolog 1; c-abl oncogene 1; receptor tyrosine kinase

tyrosine-protein kinase ABL1 isoform a; Tyrosine-protein kinase ABL1; tyrosine-protein kinase ABL1; proto-oncogene c-Abl; bcr/c-abl oncogene protein; Abelson tyrosine-protein kinase 1; c-abl oncogene 1, receptor tyrosine kinase; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolog 1; ABL proto-oncogene 1, non-receptor tyrosine kinase; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150

ABL1

ABL1; ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl; ABL; JTK7

ABL1_HUMAN

Human

1,130

This assay has high sensitivity and excellent specificity for detection of human ABL1. No significant cross-reactivity or interference between human ABL1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: 6.25 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ABL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

62362414

NP_005148.2

NM_005157.5

P00519

122,873 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CDO In Myogenesis Pathway (160998); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528)

This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014]

Abl: an ubiquitously expressed and highly conserved proto-oncogenic tyrosine kinase. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. Negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response. The Philadephia chromosome translocation t(9;22)(q34;q11) creates a Bcr-Abl fusion protein, responsible for 90% of chronic myelogenous leukemia (CML) and ~25% of acute lymphoblastic leukemia (ALL). Inhibitors: Gleevec (imatinib, Glivec), Dasatinib. Two alternatively-spliced isoforms have been described. Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; Oncoprotein; Protein kinase, TK; EC 2.7.10.2; TK group; Abl family. Chromosomal Location of Human Ortholog: 9q34.1. Cellular Component: nucleoplasm; nuclear membrane; extrinsic to internal side of plasma membrane; mitochondrion; perinuclear region of cytoplasm; leading edge; cytoplasm; nucleolus; nucleus; cytosol; actin cytoskeleton. Molecular Function: actin monomer binding; protein C-terminus binding; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; protein kinase activity; actin filament binding; protein binding; syntaxin binding; DNA binding; protein-tyrosine kinase activity; manganese ion binding; mitogen-activated protein kinase binding; nicotinate-nucleotide adenylyltransferase activity; SH3 domain binding; ATP binding; receptor binding. Biological Process: DNA damage induced protein phosphorylation; positive regulation of apoptosis; cerebellum morphogenesis; negative regulation of BMP signaling pathway; elevation of cytosolic calcium ion concentration; transitional one stage B cell differentiation; B cell receptor signaling pathway; regulation of endocytosis; negative regulation of mitotic cell cycle; neuromuscular process controlling balance; cell cycle arrest; positive regulation of oxidoreductase activity; spleen development; mitosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; muscle cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of osteoblast proliferation; negative regulation of ubiquitin-protein ligase activity; autophagy; response to oxidative stress; actin cytoskeleton organization and biogenesis; axon guidance; peptidyl-tyrosine phosphorylation; alpha-beta T cell differentiation; platelet-derived growth factor receptor signaling pathway; mitochondrial depolarization; positive regulation of mitotic cell cycle; Bergmann glial cell differentiation; regulation of transcription, DNA-dependent; collateral sprouting; cell differentiation; B cell proliferation during immune response; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; cell migration; mismatch repair; thymus development; B-1 B cell homeostasis; activated T cell proliferation; DNA damage response, signal transduction; protein modification process; regulation of cell proliferation; microspike biogenesis; innate immune response; positive regulation of muscle cell differentiation; regulation of autophagy; negative regulation of cell-cell adhesion; positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; response to DNA damage stimulus. Disease: Leukemia, Chronic Myeloid

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

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4800