catalog number :
MBS944001
products type :
ELISA Kit
products full name :
Dog Hemoglobin subunit beta, HBB ELISA Kit
products short name :
hemoglobin, beta
products name syn :
Dog Hemoglobin subunit beta (HBB) ELISA kit; CD113t-C; beta-globin; beta globin; beta globin chain; hemoglobin beta chain; hemoglobin; beta
other names :
hemoglobin subunit beta-like; Hemoglobin subunit beta; hemoglobin subunit beta-like; Beta-globin; Hemoglobin beta chain
other gene names :
LOC609402; HBB
uniprot entry name :
HBB_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of dog HBB. No significant cross-reactivity or interference between dog HBB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, lysate for RBC. Assay Type: Competitive. Detection Range: 0. 625 ug/ml -40 ug/ml. Sensitivity: The minimum detectable dose of dog HBB is typically less than 0.156 ug/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest dog HBB concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays):CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HBB. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated HBB. The competitive inhibition reaction is launched between with HRP-conjugated HBB and HBB in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of HBB in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001257812.1
ncbi gb acc num :
NM_001270883.1
ncbi mol weight :
15,996 Da
ncbi pathways :
African Trypanosomiasis Pathway (194390); African Trypanosomiasis Pathway (194323); Malaria Pathway (152669); Malaria Pathway (152657)
uniprot summary :
Function: Involved in oxygen transport from the lung to the various peripheral tissues.
size4 :
10x96-Strip-Wells