ELISA/assay

Mouse Protein Wnt-3a, WNT3A ELISA Kit

MBS943878

48-Strip-Wells

510 USD

ELISA Kit

Mouse Protein Wnt-3a, WNT3A ELISA Kit

wingless-type MMTV integration site family, member 3A

Mouse Protein Wnt-3a (WNT3A) ELISA kit; MGC119418; MGC119419; MGC119420; ; wingless-type MMTV integration site family; member 3A

protein Wnt-3a; Protein Wnt-3a; protein Wnt-3a; vestigial tail; wingless-related MMTV integration site 3A; wingless-type MMTV integration site family, member 3A

WNT3A

Wnt3a; Wnt3a; vt; Wnt-3a; Wnt-3a

WNT3A_MOUSE

Mouse

352

This assay has high sensitivity and excellent specificity for detection of mouse WNT3A. No significant cross-reactivity or interference between mouse WNT3A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of mouse WNT3A is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT3A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

7106447

NP_033548.1

NM_009522.2

P27467

39,258 Da

Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (575448); ESC Pluripotency Pathways (198374); GPCR Ligand Binding Pathway (575425); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Melanogenesis Pathway (83289)

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; extracellular region. Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; receptor binding; frizzled-2 binding. Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell proliferation in forebrain; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of receptor internalization; positive regulation of caspase activity; positive regulation of collateral sprouting in the absence of injury; Wnt receptor signaling pathway through beta-catenin; palate development; regulation of axonogenesis; signal transduction; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; BMP signaling pathway; anterior/posterior pattern formation; mammary gland development; cell-cell signaling; regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of B cell proliferation; somatic stem cell division; mesoderm development; hemopoiesis; heart looping; dorsoventral neural tube patterning; positive regulation of cytokine production; platelet activation; somitogenesis; inner ear morphogenesis; Wnt receptor signaling pathway; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; organ morphogenesis; axonogenesis; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800