catalog number :
MBS943749
products type :
ELISA Kit
products full name :
Human Bone morphogenetic protein 3, BMP3 ELISA Kit
products short name :
bone morphogenetic protein 3
products name syn :
Human Bone morphogenetic protein 3 (BMP3) ELISA kit; BMP-3A; bone morphogenetic protein 3 (osteogenic) ; bone morphogenetic protein-3; osteogenin; bone morphogenetic protein 3
other names :
bone morphogenetic protein 3 preproprotein; Bone morphogenetic protein 3; bone morphogenetic protein 3; BMP-3; osteogenin; bone morphogenetic protein-3; bone morphogenetic protein 3A; bone morphogenetic protein 3 (osteogenic); bone morphogenetic protein 3; Bone morphogenetic protein 3A; BMP-3A; Osteogenin
products gene name :
BMP3
other gene names :
BMP3; BMP3; BMP-3A; BMP3A; BMP-3; BMP-3A
uniprot entry name :
BMP3_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human BMP3. No significant cross-reactivity or interference between Human BMP3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Chondrogenesis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001192.2
ncbi gb acc num :
NM_001201.2
ncbi mol weight :
53,372 Da
ncbi pathways :
Adipogenesis Pathway (198832)
ncbi summary :
BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein, also known as osteogenin, induces bone formation. [provided by RefSeq, Jul 2008]
uniprot summary :
BMP3: Negatively regulates bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 4q21. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding. Biological Process: regulation of apoptosis; ossification; cell-cell signaling; cartilage development; regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; skeletal development; cell development; growth
size4 :
10x96-Strip-Wells