ELISA/assay

Human Filamin-A, FLNA ELISA Kit

MBS943682

48-Strip-Wells

510 USD

ELISA Kit

Human Filamin-A, FLNA ELISA Kit

filamin A, alpha (actin binding protein 280)

Human Filamin-A (FLNA) ELISA kit; XX-FW83128A1.1; ABP-280; ABPX; DKFZp434P031; FLN; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; actin binding protein 280; endothelial actin-binding protein; filamin 1; non-muscle filamin; filamin A; alpha (actin binding protein 280)

filamin-A isoform 2; Filamin-A; filamin-A; filamin-1; alpha-filamin; non-muscle filamin; actin binding protein 280; endothelial actin-binding protein; filamin A, alpha; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin

FLNA

FLNA; FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280; FLN; FLN1; FLN-A; ABP-280

FLNA_HUMAN

Human

2,647

This assay has high sensitivity and excellent specificity for detection of Human FLNA. No significant cross-reactivity or interference between Human FLNA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.68 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FLNA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FLNA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FLNA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLNA bound in the initial step. The color development is stopped and the intensity of the color is measured.

160420317

NP_001104026.1

NM_001110556.1

P21333

280,739 Da

Androgen Receptor Signaling Pathway (198806); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-extracellular Matrix Interactions Pathway (160967); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); GP1b-IX-V Activation Signalling Pathway (187205); Hemostasis Pathway (106028); MAPK Signaling Pathway (198779)

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Protein type: Nuclear receptor co-regulator; Actin-binding; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus. Molecular Function: actin filament binding; small GTPase binding; signal transducer activity; protein binding; Rho GTPase binding; protein homodimerization activity; protein kinase C binding; mu-type opioid receptor binding; Rac GTPase binding; Ral GTPase binding; glycoprotein binding; SMAD binding; transcription factor binding. Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; receptor clustering; mRNA transcription from RNA polymerase II promoter; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; positive regulation of transcription factor import into nucleus; cytoplasmic sequestering of protein; cilium biogenesis; epithelial to mesenchymal transition; blood coagulation; negative regulation of protein catabolic process. Disease: Terminal Osseous Dysplasia; Fg Syndrome 2; Frontometaphyseal Dysplasia; Cardiac Valvular Dysplasia, X-linked; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800