ELISA/assay

Mouse Regenerating islet-derived protein 3-gamma, REG3G ELISA Kit

MBS943610

48-Strip-Wells

510 USD

ELISA Kit

Mouse Regenerating islet-derived protein 3-gamma, REG3G ELISA Kit

regenerating islet-derived 3 gamma

Mouse Regenerating islet-derived protein 3-gamma (REG3G) ELISA kit; MGC118998; MGC118999; MGC119001; PAP1B; PAPIB; REG-III; UNQ429; LPPM429; PAP IB protein; regenerating gene III; regenerating islet-derived 3 gamma

regenerating islet-derived protein 3-gamma; Regenerating islet-derived protein 3-gamma; regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; RegIII (gamma); pancreatitis-associated protein 3; regenerating islet-derived protein III-gamma; generating islet-derived, mouse homolog 3 gamma; regenerating islet-derived 3 gamma; Pancreatitis-associated protein 3; Regenerating islet-derived protein III-gamma; Reg III-gamma

REG3G

Reg3g; Reg3g; AI449515; Pap3; REG-3-gamma; Reg III-gamma

REG3G_MOUSE

Mouse

174

This assay has high sensitivity and excellent specificity for detection of mouse REG3G. No significant cross-reactivity or interference between mouse REG3G and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse REG3G is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for REG3G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG3G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG3G is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG3G bound in the initial step. The color development is stopped and the intensity of the color is measured.

6755310

NP_035390.1

NM_011260.1

O09049

19,307 Da

Function: Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.7 PublicationsManual assertion based on experiment in:Ref.3

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800