catalog number :
MBS943610
products type :
ELISA Kit
products full name :
Mouse Regenerating islet-derived protein 3-gamma, REG3G ELISA Kit
products short name :
regenerating islet-derived 3 gamma
products name syn :
Mouse Regenerating islet-derived protein 3-gamma (REG3G) ELISA kit; MGC118998; MGC118999; MGC119001; PAP1B; PAPIB; REG-III; UNQ429; LPPM429; PAP IB protein; regenerating gene III; regenerating islet-derived 3 gamma
other names :
regenerating islet-derived protein 3-gamma; Regenerating islet-derived protein 3-gamma; regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; RegIII (gamma); pancreatitis-associated protein 3; regenerating islet-derived protein III-gamma; generating islet-derived, mouse homolog 3 gamma; regenerating islet-derived 3 gamma; Pancreatitis-associated protein 3; Regenerating islet-derived protein III-gamma; Reg III-gamma
products gene name :
REG3G
other gene names :
Reg3g; Reg3g; AI449515; Pap3; REG-3-gamma; Reg III-gamma
uniprot entry name :
REG3G_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse REG3G. No significant cross-reactivity or interference between mouse REG3G and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse REG3G is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for REG3G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG3G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG3G is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG3G bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_035390.1
ncbi gb acc num :
NM_011260.1
ncbi mol weight :
19,307 Da
uniprot summary :
Function: Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.7 PublicationsManual assertion based on experiment in:Ref.3
size4 :
10x96-Strip-Wells