Human Tripartite motif-containing protein 72, TRIM72 ELISA Kit | MyBioSource MBS943592 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tripartite motif-containing protein 72, TRIM72 ELISA Kit

catalog :

MBS943592

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS943592

products type :

ELISA Kit

products full name :

Human Tripartite motif-containing protein 72, TRIM72 ELISA Kit

products short name :

tripartite motif-containing 72

products name syn :

Human Tripartite motif-containing protein 72 (TRIM72) ELISA kit; MG53; mitsugumin-53; tripartite motif-containing 72

other names :

tripartite motif-containing protein 72; Tripartite motif-containing protein 72; tripartite motif-containing protein 72; mitsugumin 53; mitsugumin-53; tripartite motif-containing 72; tripartite motif containing 72, E3 ubiquitin protein ligase; Mitsugumin-53; Mg53

products gene name :

TRIM72

other gene names :

TRIM72; TRIM72; MG53; MG53; Mg53

uniprot entry name :

TRI72_HUMAN

reactivity :

Human

sequence length :

477

specificity :

This assay has high sensitivity and excellent specificity for detection of human TRIM72. No significant cross-reactivity or interference between human TRIM72 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml-1600 pg/ml. Sensitivity: The minimum detectable dose of human TRIM72 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRIM72 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRIM72 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM72 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM72 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

270265876

ncbi acc num :

NP_001008275.2

ncbi gb acc num :

NM_001008274.3

uniprot acc num :

Q6ZMU5

ncbi mol weight :

52,731 Da

uniprot summary :

TRIM72: Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system; Ubiquitin ligase. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: cytoplasmic vesicle membrane; sarcolemma. Molecular Function: zinc ion binding; phosphatidylserine binding. Biological Process: muscle development; exocytosis; plasma membrane repair; muscle system process; protein homooligomerization

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage