catalog number :
MBS943564
products type :
ELISA Kit
products full name :
Bovine C-X-C motif chemokine 10, CXCL10 ELISA Kit
products short name :
chemokine (C-X-C motif) ligand 10
products name syn :
Bovine C-X-C motif chemokine 10 (CXCL10) ELISA kit; C7; IFI10; INP10; IP-10; SCYB10; crg-2; gIP-10; mob-1; gamma IP10; interferon-inducible cytokine IP-10; protein 10 from interferon (gamma) -induced cell line; small inducible cytokine B10; small inducibl; chemokine (C-X-C motif) ligand 10
other names :
C-X-C motif chemokine 10; C-X-C motif chemokine 10; C-X-C motif chemokine 10; small-inducible cytokine B10; gamma interferon inducible protein 10; chemokine (C-X-C motif) ligand 10; Small-inducible cytokine B10
products gene name :
CXCL10
other gene names :
CXCL10; CXCL10
uniprot entry name :
CXL10_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine CXCL10. No significant cross-reactivity or interference between Bovine CXCL10 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCL10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001040016.1
ncbi gb acc num :
NM_001046551.2
ncbi mol weight :
11,427 Da
ncbi pathways :
Chemokine Receptors Bind Chemokines Pathway (581438); Chemokine Signaling Pathway (98761); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (581436); Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125127); Cytosolic DNA-sensing Pathway (124833); G Alpha (i) Signalling Events Pathway (581463); GPCR Downstream Signaling Pathway (581461)
uniprot summary :
Function: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3
size4 :
10x96-Strip-Wells