Human R-spondin-1, RSPO1 ELISA Kit | MyBioSource MBS943519 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human R-spondin-1, RSPO1 ELISA Kit

catalog :

MBS943519

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS943519

products type :

ELISA Kit

products full name :

Human R-spondin-1, RSPO1 ELISA Kit

products short name :

R-spondin homolog (Xenopus laevis)

products name syn :

Human R-spondin-1 (RSPO1) ELISA kit; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO; OTTHUMP00000180612; OTTHUMP00000180613; R-spondin1; roof plate-specific spondin; R-spondin homolog (Xenopus laevis)

other names :

R-spondin-1 isoform 1; R-spondin-1; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1; R-spondin 1; Roof plate-specific spondin-1; hRspo1

products gene name :

RSPO1

other gene names :

RSPO1; RSPO1; RSPO; CRISTIN3; hRspo1

uniprot entry name :

RSPO1_HUMAN

reactivity :

Human

sequence length :

263

specificity :

This assay has high sensitivity and excellent specificity for detection of Human RSPO1. No significant cross-reactivity or interference between Human RSPO1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Neurobiology. Protein Biological Process 3: Sensory transduction

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RSPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

84490388

ncbi acc num :

NP_001033722.1

ncbi gb acc num :

NM_001038633.3

uniprot acc num :

Q2MKA7

ncbi mol weight :

28,959 Da

ncbi pathways :

Wnt Signaling Network Pathway (137962)

ncbi summary :

This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

uniprot summary :

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Cell development/differentiation; Cell adhesion; Secreted. Chromosomal Location of Human Ortholog: 1p34.3. Cellular Component: extracellular space; extracellular region; nucleus. Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding. Biological Process: regulation of gene expression; regulation of receptor internalization; positive regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway through beta-catenin; male meiosis; positive regulation of Wnt receptor signaling pathway. Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage