ELISA/assay

Pig glucagon like peptide 1, GLP1 ELISA Kit

MBS943508

48-Strip-Wells

565 USD

ELISA Kit

Pig glucagon like peptide 1, GLP1 ELISA Kit

glucagon like peptide 1 (GLP1)

Pig glucagon like peptide 1 (GLP1) ELISA Kit; glucagon like peptide 1 (GLP1)

GLP1; Putative uncharacterized protein FP13812; histone-lysine N-methyltransferase EHMT1; H3-K9-HMTase 5; G9a-like protein 1; lysine N-methyltransferase 1D; histone H3-K9 methyltransferase 5; histone-lysine N-methyltransferase, H3 lysine-9 specific 5; euchromatic histone-lysine N-methyltransferase 1; Putative uncharacterized protein FP13812

GLP1

EHMT1; FP13812; GLP; GLP1; KMT1D; FP13812; EUHMTASE1; Eu-HMTase1; bA188C12.1

Q71M33_HUMAN

Pig

917

This assay has high sensitivity and excellent specificity for detection of pig GLP1. No significant cross-reactivity or interference between pig GLP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

14211561

BAB56104.1

Q71M33

18,232 Da

Lysine Degradation Pathway (82956); Lysine Degradation Pathway (320)

The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

EHMT1: Histone methyltransferase that specifically mono- and dimethylates Lys-9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates Lys-27 of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of Lys-373 of p53/TP53. Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome). Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems. Belongs to the histone-lysine methyltransferase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine. Chromosomal Location of Human Ortholog: 9q34.3. Cellular Component: nucleoplasm; cytoplasm; plasma membrane; chromosome; nucleus. Molecular Function: methyltransferase activity; protein binding; zinc ion binding; p53 binding; protein-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific). Biological Process: histone methylation; embryonic development; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; DNA methylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; histone H3-K9 methylation. Disease: Kleefstra Syndrome

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150