catalog number :
MBS943465
products type :
ELISA Kit
products full name :
Human Statherin, STATH ELISA Kit
products short name :
statherin
products name syn :
Human Statherin (STATH) ELISA kit; STR; ; statherin
other names :
statherin isoform b; Statherin; statherin; statherin
products gene name :
STATH
other gene names :
STATH; STATH; STR
uniprot entry name :
STAT_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human STATH. No significant cross-reactivity or interference between human STATH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, saliva. Assay Type: Sandwich. Detection Range: 78 ng/ml -5000 ng/ml. Sensitivity: The minimum detectable dose of human STATH is typically less than 19.5 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for STATH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STATH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STATH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STATH bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001009181.1
ncbi gb acc num :
NM_001009181.1
ncbi mol weight :
7,304 Da
ncbi pathways :
Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)
uniprot summary :
STATH: Salivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth surface. Belongs to the histatin/statherin family. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular region. Molecular Function: protein binding; hydroxyapatite binding; structural constituent of tooth enamel; extracellular matrix constituent, lubricant activity. Biological Process: negative regulation of bone mineralization; ossification; biomineral formation; saliva secretion
size4 :
10x96-Strip-Wells