ELISA/assay

Human Telomerase reverse transcriptase, TERT ELISA Kit

MBS943198

96 Strip Wells

760 USD

ELISA Kit

Human Telomerase reverse transcriptase, TERT ELISA Kit

telomerase reverse transcriptase

Human Telomerase reverse transcriptase (TERT) ELISA kit; EST2; TCS1; TP2; TRT; hEST2; telomerase catalytic subunit; telomerase reverse transcriptase

telomerase reverse transcriptase isoform 2; Telomerase reverse transcriptase; telomerase reverse transcriptase; telomerase catalytic subunit; telomerase-associated protein 2; telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2; TP2

TERT

TERT; TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1; EST2; TCS1; TRT; TP2

TERT_HUMAN

Human

1069

This assay has high sensitivity and excellent specificity for detection of Human TERT. No significant cross-reactivity or interference between Human TERT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates. Detection Range: 6.25 ng/ml-400 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 1.56 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TERT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TERT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TERT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TERT bound in the initial step. The color development is stopped and the intensity of the color is measured.

301129200

NP_001180305.1

NM_001193376.1

O14746

126,997 Da

Cell Cycle Pathway 530733!!Chromosome Maintenance Pathway 161048!!Extension Of Telomeres Pathway 106541!!HIF-1-alpha Transcription Factor Network Pathway 138045!!HTLV-I Infection Pathway 373901!!HTLV-I Infection Pathway 373889!!IL-2 Signaling Pathway 198885!!IL2 Signaling Events Mediated By PI3K Pathway 137938!!Id Signaling Pathway 198871!!Regulation Of Telomerase Pathway 137987

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]

Function: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.12 PublicationsManual assertion based on experiment in:Ref.11. Catalytic activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

96 Strip Wells

760 USD