catalog number :
MBS943195
products type :
ELISA Kit
products full name :
Mouse Dickkopf-related protein 3, DKK3 ELISA Kit
products short name :
[dickkopf homolog 3 (Xenopus laevis)]
products name syn :
[Mouse Dickkopf-related protein 3 (DKK3) ELISA kit; REIC; RIG; RIG-like 5-6; RIG-like 7-1; dickkopf 3; dickkopf homolog 3; regulated in glioma; dickkopf homolog 3 (Xenopus laevis)]
other names :
[dickkopf-related protein 3; Dickkopf-related protein 3; dickkopf-related protein 3; dkk-3; mDkk-3; dickkopf-3; dickkopf homolog 3 (Xenopus laevis)]
products gene name :
[DKK3]
other gene names :
[Dkk3; Dkk3; C87148; AW061014; Dickkopf-3; Dkk-3; mDkk-3]
uniprot entry name :
DKK3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse DKK3. No significant cross-reactivity or interference between mouse DKK3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 9.4 pg/ml -600 pg/ml. Sensitivity: <2.35 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DKK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DKK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DKK3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DKK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_056629.1
ncbi gb acc num :
NM_015814.2
ncbi mol weight :
38,388 Da
uniprot summary :
DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; extracellular region. Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; multicellular organismal development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells