Mouse Disintegrin and metalloproteinase domain-containing protein 15, ADAM15 ELISA Kit | MyBioSource MBS943150 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Disintegrin and metalloproteinase domain-containing protein 15, ADAM15 ELISA Kit

catalog :

MBS943150

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS943150

products type :

ELISA Kit

products full name :

Mouse Disintegrin and metalloproteinase domain-containing protein 15, ADAM15 ELISA Kit

products short name :

ADAM metallopeptidase domain 15

products name syn :

Mouse Disintegrin and metalloproteinase domain-containing protein 15 (ADAM15) ELISA kit; RP11-540D14.1; MDC15; a disintegrin and metalloproteinase domain 15; a disintegrin and metalloproteinase domain 15 (metargidin) ; metalloprotease RGD disintegrin protein; metalloproteinase-like; disinte; ADAM metallopeptidase domain 15

other names :

disintegrin and metalloproteinase domain-containing protein 15 isoform a; Disintegrin and metalloproteinase domain-containing protein 15; disintegrin and metalloproteinase domain-containing protein 15; AD56; MDC-15; ADAM 15; disintegrin and metalloprotease 15; metalloprotease RGD disintegrin protein; a disintegrin and metalloprotease domain 15 (metargidin); a disintegrin and metalloproteinase domain 15 (metargidin); metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; a disintegrin and metallopeptidase domain 15 (metargidin); AD56; Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin

products gene name :

ADAM15

other gene names :

Adam15; Adam15; MDC15; metargidin; Mdc15; ADAM 15; MDC-15

uniprot entry name :

ADA15_MOUSE

reactivity :

Mouse

sequence length :

864

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse ADAM15. No significant cross-reactivity or interference between mouse ADAM15 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: 4.69 pg /ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

109148517

ncbi acc num :

NP_001032811.2

ncbi gb acc num :

NM_001037722.2

uniprot acc num :

O88839

ncbi mol weight :

92,664 Da

uniprot summary :

ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Protease; Motility/polarity/chemotaxis; Cell adhesion; EC 3.4.24.-. Cellular Component: cell surface; cell projection; membrane; integral to membrane; cytoplasmic vesicle; intracellular; cell junction. Molecular Function: peptidase activity; integrin binding; protein binding; metallopeptidase activity; hydrolase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding; SH3 domain binding. Biological Process: integrin-mediated signaling pathway; collagen catabolic process; innate immune response; negative regulation of cell growth; angiogenesis; negative regulation of cell-matrix adhesion; cell adhesion; proteolysis; negative regulation of cell migration

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage