Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, ATP2A3 ELISA Kit | MyBioSource MBS943076 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, ATP2A3 ELISA Kit

catalog :

MBS943076

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS943076

products type :

ELISA Kit

products full name :

Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, ATP2A3 ELISA Kit

products short name :

ATPase, Ca++ transporting, ubiquitous

products name syn :

Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 (ATP2A3) ELISA kit; SERCA3; ATPase; Ca (2+) -transporting; ubiquitous; SR Ca (2+) -ATPase 3; adenosine triphosphatase; calcium; calcium pump 3; calcium-translocating P-type ATPase; sarco/endoplasmic reticulum Ca2+ -ATPase; sarco; ATPase; Ca++ transporting; ubiquitous

other names :

sarcoplasmic/endoplasmic reticulum calcium ATPase 3 isoform a; Sarcoplasmic/endoplasmic reticulum calcium ATPase 3; sarcoplasmic/endoplasmic reticulum calcium ATPase 3; calcium pump 3; SR Ca(2+)-ATPase 3; adenosine triphosphatase, calcium; calcium-translocating P-type ATPase; ATPase, Ca(2+)-transporting, ubiquitous; sarco/endoplasmic reticulum Ca2+ -ATPase; ATPase, Ca++ transporting, ubiquitous; Calcium pump 3

products gene name :

ATP2A3

other gene names :

ATP2A3; ATP2A3; SERCA3; SERCA3; SR Ca(2+)-ATPase 3

uniprot entry name :

AT2A3_HUMAN

reactivity :

Human

sequence length :

999

specificity :

This assay has high sensitivity and excellent specificity for detection of human ATP2A3. No significant cross-reactivity or interference between human ATP2A3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human ATP2A3 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATP2A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATP2A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATP2A3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATP2A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

28373103

ncbi acc num :

NP_005164.2

ncbi gb acc num :

NM_005173.3

uniprot acc num :

Q93084

ncbi mol weight :

113,977 Da

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Hemostasis Pathway (106028); Ion Channel Transport Pathway (187193); Ion Transport By P-type ATPases Pathway (187194); Myometrial Relaxation And Contraction Pathways (198759); Pancreatic Secretion Pathway (169306)

ncbi summary :

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

ATP2A3: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 7 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.6.3.8; Endoplasmic reticulum; Hydrolase; Transporter, ion channel; Membrane protein, integral; Transporter; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 17p13.3. Cellular Component: endoplasmic reticulum membrane; nuclear membrane; sarcoplasmic reticulum membrane; sarcoplasmic reticulum; endoplasmic reticulum; integral to membrane. Molecular Function: calcium-transporting ATPase activity; metal ion binding; ATP binding. Biological Process: transport; metabolic process; calcium ion transport; blood coagulation; transmembrane transport

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage