catalog number :
MBS943055
products type :
ELISA Kit
products full name :
Mouse Vesicular inhibitory amino acid transporter, SLC32A1 ELISA Kit
products short name :
solute carrier family 32 (GABA vesicular transporter) , member 1
products name syn :
Mouse Vesicular inhibitory amino acid transporter (SLC32A1) ELISA kit; VGAT; VIAAT; GABA and glycine transporter; solute carrier family 32; member 1; vesicular GABA transporter; vesicular inhibitory amino acid transporter; solute carrier family 32 (GABA vesicular transporter); member 1
other names :
vesicular inhibitory amino acid transporter; Vesicular inhibitory amino acid transporter; vesicular inhibitory amino acid transporter; mVGAT; mVIAAT; vesicular GABA transporter; GABA and glycine transporter; solute carrier family 32 member 1; vasicular inhibitory amino acid transporter 10D; solute carrier family 32 (GABA vesicular transporter), member 1; GABA and glycine transporter; Solute carrier family 32 member 1; Vesicular GABA transporter; mVGAT; mVIAAT
products gene name :
SLC32A1
other gene names :
Slc32a1; Slc32a1; VGAT; Viaat; R75019; Vgat; Viaat; mVGAT; mVIAAT
uniprot entry name :
VIAAT_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse SLC32A1. No significant cross-reactivity or interference between mouse SLC32A1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 7.81 pg/ml-500 pg/ml. Sensitivity: The minimum detectable dose of mouse SLC32A1 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SLC32A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC32A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC32A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC32A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033534.2
ncbi gb acc num :
NM_009508.2
ncbi mol weight :
57,381 Da
ncbi pathways :
GABAergic Synapse Pathway (377269); GABAergic Synapse Pathway (377129); Morphine Addiction Pathway (552689); Morphine Addiction Pathway (554808); Nicotine Addiction Pathway (583269); Nicotine Addiction Pathway (584676); Retrograde Endocannabinoid Signaling Pathway (537783); Retrograde Endocannabinoid Signaling Pathway (539806); SLC-mediated Transmembrane Transport Pathway (575016); Synaptic Vesicle Cycle Pathway (373902)
uniprot summary :
SLC32A1: Involved in the uptake of GABA and glycine into the synaptic vesicles. Belongs to the amino acid/polyamine transporter 2 family. Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter. Cellular Component: synaptic vesicle; neuron projection; membrane; integral to plasma membrane; dendrite; presynaptic active zone; integral to membrane; intracellular organelle; synapse; cytoplasmic vesicle. Molecular Function: glycine transmembrane transporter activity; gamma-aminobutyric acid:hydrogen symporter activity. Biological Process: glycine transport; proton transport; transport; neurotransmitter transport; gamma-aminobutyric acid transport
size4 :
10x96-Strip-Wells